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KMID : 0613820070170070896
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Journal of Life Science
2007 Volume.17 No. 7 p.896 ~ p.904
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Induction of Apoptosis by Vitamin E Succinate in Human Erythroleukemia K562 Cells
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Jang Chang-Deug
Park Hae-Ryoun
Kim Jong-Myoung
An Won-Gun
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Abstract
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Regulation mechanism of apoptosis has been known to be important for understanding the pathogenesis of a number of human diseases including cancers. The effects of RRR-α-tocopheryl succinate (vitamin E succinate, VES) on the cell viability, generation of ROS, expression of proteins involved in apoptosis, and growth of human chronic myelogenous leukemia K562 cells were analyzed in this study. VES treatment not only induced the generation of the ROS but also increased the levels of NF-κ B, COX-2, and p21WAF1/CIP1 in K562 cells. It modulates the levels of pro-apoptotic proteins such as Bax provoking the apoptosis in K562 cells. The cleavage of PARP into 89 kDa was also increased upon VES treatment in a dosage-dependent manner. Induction of an apoptosis was evident by the increase of sub-G1 peak and cell shrinkage condensed chromatin in K562 cells treated with VES. It also resulted in an inhibition of tumor growth by 50% and prolonged survival of the lymphoma-induced mice. This potentiation of VES obtained in vitro and in vivo may indicate the feasibility of more effective chemotherapy in chronic myelogenous leukemia.
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KEYWORD
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VES(Vitamin E succinate), apoptosis, cancer
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